Single-cell & spatial profiling
Reading the genes of each individual cell, and mapping where each cell sits in the tumour.
scRNA-seq (10x Genomics) resolves tumour, immune, and stromal populations; spatial transcriptomics (Visium, Xenium, CosMx, MERFISH) and multiplex protein imaging (CODEX, IMC) keep tissue architecture. Revealing how ADC bystander killing, T-cell exclusion, and resistance niches work. Research-grade; entering trials as correlative science.
How it works
RNA is captured with barcodes per cell or per spatial location, or read by imaging-based in situ hybridisation for hundreds to thousands of genes.
- Resolves heterogeneity and microenvironment
- Discovery engine for new targets
- Cost, throughput, analysis burden
- Not yet clinically actionable
Latest papers
topQuery for this technology: (TITLE:"single-cell RNA sequencing" OR ABSTRACT:"single-cell RNA sequencing" OR TITLE:"spatial transcriptomics" OR ABSTRACT:"spatial transcriptomics" OR TITLE:"single-cell" OR ABSTRACT:"single-cell") AND (cancer OR tumor OR tumour OR oncology OR carcinoma OR lymphoma OR leukemia OR leukaemia OR myeloma OR sarcoma OR melanoma OR glioma). Results are unfiltered search hits about Single-cell & spatial profiling, not a curated reading list.
Pages like this
not linked directly; found by shared links- TermHot vs cold tumours
Shares Miriam Merad, Map which tumour clones sit next to which immune cells before choosing therapy, Standards for spatial and multiplex tissue biomarkers before they reach the clinic, Clear the suppressive neutrophils out of pancreatic tumours first.
- TermTumour-infiltrating lymphocytes (TILs)
Shares Map which tumour clones sit next to which immune cells before choosing therapy, Standards for spatial and multiplex tissue biomarkers before they reach the clinic, Spatial-omics-guided treatment selection, Grow immune command posts inside tumours.
- TargetFAP
Shares Block the survival signals the tumour's neighbours provide, Targeting tumour mechanics and pressure, Match therapy to the type of scar-forming cell in the tumour, Make every cold tumour hot: a coordinated programme to reprogramme immune-excluded tumours.
- TermDesmoplasia (tumour stroma)
Shares Targeting tumour mechanics and pressure, Clear the suppressive neutrophils out of pancreatic tumours first, Match therapy to the type of scar-forming cell in the tumour, Invasion: proteases, adhesion & the invasive front.
- TechnologyWhole-exome & whole-genome sequencing
Shares Valius Sciences, A national rapid research autopsy network for end-stage cancer, Wellcome, A global rapid tissue donation network for metastatic disease.
- BottleneckNo one can predict who responds to immunotherapy
Shares Map which tumour clones sit next to which immune cells before choosing therapy, Standards for spatial and multiplex tissue biomarkers before they reach the clinic, Clear the suppressive neutrophils out of pancreatic tumours first, Reprogramme suppressive macrophages instead of trying to delete them.
- PathwayTGF-β signalling
Shares What actually holds T cells at the tumour border?, Invasion: proteases, adhesion & the invasive front, The metastatic cascade, Fibroblast activation, desmoplasia & matrix stiffness.
- TechnologyCRISPR functional genomics
Shares State (Arc Institute perturbation model), An open model of every cancer cell state, built from perturbation atlases, Institut Pasteur, An open foundation model of the cancer cell trained on perturbation data.