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Functional (ex vivo) drug testing

Growing a patient's own cancer cells in a dish and testing drugs on them directly, instead of guessing from genetics.

Patient-derived organoids and short-term ex vivo cultures (SEngine PARIS, Cancertain, Xilis, Curesponse) expose live tumour cells to drug panels. Feasibility and correlation with response are established in colorectal, pancreatic, and ovarian cancer; randomised proof of benefit is pending. Complementary to genomics when no actionable mutation exists.

Schematic · not to scale
Patient tumour cells · Drug per well → viability

How it works

Fresh tumour tissue dissociated and cultured in matrix; viability read-outs per drug within 1-3 weeks.

Strengths
  • Phenotype captures what genotype misses
  • Tests combinations
Limitations
  • Take rate and timeline
  • No stroma or immune component in most systems

Key papers

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Latest papers

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Literature trend145 papers in the last 12 months+42% vs prior 12How this is computed
Latest papers · live from Europe PMC
Open in Europe PMC

Query for this technology: (TITLE:"functional precision medicine" OR ABSTRACT:"functional precision medicine" OR TITLE:"ex vivo drug sensitivity" OR ABSTRACT:"ex vivo drug sensitivity" OR TITLE:"drug sensitivity testing" OR ABSTRACT:"drug sensitivity testing") AND (cancer OR tumor OR tumour OR oncology OR carcinoma OR lymphoma OR leukemia OR leukaemia OR myeloma OR sarcoma OR melanoma OR glioma). Results are unfiltered search hits about Functional (ex vivo) drug testing, not a curated reading list.

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