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RFdiffusion / RFdiffusion2 and ProteinMPNN (Baker Lab)

The tools that design entirely new proteins to bind a chosen target, now used for cancer binders and antibodies.

RFdiffusion (Nature 2023) generates protein backbones; ProteinMPNN designs sequences; RFdiffusion2 (2025) designs enzymes; RFantibody extends to antibodies. Basis of Xaira and many binder programmes.

Generic schematic · not to scale · placeholder for the drug discovery front
Guide RNA library · Knockout → dependency

How it works

Denoising diffusion over protein backbones conditioned on a target.

Strengths
  • Validated de novo binders
Limitations
  • Developability and immunogenicity still empirical
Since
2023

Latest papers

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Latest papers · live from Europe PMC
Open in Europe PMC

Query for this technology: (TITLE:"RFdiffusion / RFdiffusion2 and ProteinMPNN" OR ABSTRACT:"RFdiffusion / RFdiffusion2 and ProteinMPNN" OR TITLE:"Baker Lab" OR ABSTRACT:"Baker Lab") AND (cancer OR tumor OR tumour OR oncology OR carcinoma OR lymphoma OR leukemia OR leukaemia OR myeloma OR sarcoma OR melanoma OR glioma). Results are unfiltered search hits about RFdiffusion / RFdiffusion2 and ProteinMPNN (Baker Lab), not a curated reading list.

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