Plasmid DNA and mRNA raw-material manufacturing
Plasmid DNA and mRNA raw materials are the DNA templates and enzymes behind viral vectors and mRNA vaccines. They are invisible to patients but decisive for supply.
GMP plasmid DNA is the starting material for lentiviral vectors and for in vitro transcription of mRNA (personalised neoantigen vaccines, in vivo CAR). Supply tightened during the pandemic and again with the growth of individualised mRNA, prompting dedicated suppliers (Aldevron/Danaher, VGXI, Cobra) and cell-free synthetic DNA alternatives (Touchlight's doggybone DNA).
How it works
Bacterial fermentation and purification of high-copy plasmids, or enzymatic cell-free amplification; linearised template drives IVT with modified nucleosides.
- Well-characterised
- Scalable fermentation
- Weeks of lead time
- Endotoxin and supercoiling specifications
- Single-source risk for GMP grades
Latest papers
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