Multiparameter flow cytometry MRD
Flow cytometry MRD counts leukaemia cells in the bone marrow one at a time by their surface proteins, down to one in ten thousand.
Eight- to ten-colour flow detects leukaemia-associated immunophenotypes or 'different-from-normal' patterns at 10^-4 sensitivity. The most widely available MRD method in AML and ALL; ELN 2021 MRD guidelines define thresholds and timepoints. Next-generation flow (EuroFlow) reaches 10^-5 in myeloma and ALL. In CLL, undetectable MRD (<10^-4) after venetoclax combinations predicts long remission and is a regulatory endpoint.
How it works
Panels of fluorescently tagged antibodies distinguish aberrant blasts from regenerating normal precursors.
- Broadly applicable, fast (same day)
- Works without a molecular marker
- Operator and lab dependent
- Sensitivity below molecular methods; immunophenotype shift after therapy
Latest papers
topQuery for this technology: (TITLE:"Multiparameter flow cytometry MRD" OR ABSTRACT:"Multiparameter flow cytometry MRD") AND (cancer OR tumor OR tumour OR oncology OR carcinoma OR lymphoma OR leukemia OR leukaemia OR myeloma OR sarcoma OR melanoma OR glioma). Results are unfiltered search hits about Multiparameter flow cytometry MRD, not a curated reading list.
Pages like this
not linked directly; found by shared links- TechnologyNGS-based MRD (clonoSEQ and molecular MRD)
Shares MRD-negative complete remission, Myelodysplastic syndromes / neoplasms (MDS), Dormant cells and minimal residual disease, Minimal / molecular residual disease (MRD).
- InstitutionSylvester Comprehensive Cancer Center, University of Miami
Shares Myelodysplastic syndromes / neoplasms (MDS), Minimal / molecular residual disease (MRD), MRD / molecular residual disease testing, Multiple myeloma.
- CompanyAdaptive Biotechnologies
Shares Dormant cells and minimal residual disease, Chronic lymphocytic leukaemia, MRD / molecular residual disease testing, Multiple myeloma.
- InstitutionGIMEMA
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, MRD / molecular residual disease testing, Multiple myeloma.
- CollectionLeukemia & Lymphoma Society (LLS)
Shares Myelodysplastic syndromes / neoplasms (MDS), Chronic lymphocytic leukaemia, Multiple myeloma, Acute lymphoblastic leukaemia.
- TermFlow cytometry (immunophenotyping)
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, Multiple myeloma, Acute lymphoblastic leukaemia.
- InstitutionEuropean Hematology Association
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, MRD / molecular residual disease testing, Multiple myeloma.
- TermUndetectable MRD (uMRD / MRD-negative)
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, MRD / molecular residual disease testing, Multiple myeloma.